Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Pipettor Head Kit, 8-Channel, 96-Well, Tips Included
The Molecular Devices FlexStation 3 Pipettor Head Kit supplies an integrated 8-channel pipettor and starter consumables to enable automated liquid handling on FlexStation 3 multi-mode microplate readers. It is intended for 96-well assay workflows, supporting compound additions and real-time kinetic measurements.
- 8-channel pipettor
- Ten racks of 96-well pipet tips, black
- One 96-well yellow plate
- Designed for automated liquid handling of 96-well assays
- Supports real-time kinetic compound additions
Molecular Devices Fluorescence Polarization Filter, 635 nm, Emission
Fluorescence polarization emission filter centered at 635 nm for use with SpectraMax iD5 microplate readers. The filter is intended for fluorescence polarization assays and mounts in the instrument filter slide for straightforward exchange.
- Provides emission detection centered at 635 nm for red-shifted fluorophores.
- Designed for fluorescence polarization measurements.
- Fits 25 mm filter slides used in SpectraMax iD5 readers.
- Installs into the instrument filter slide for tool-free exchange.
- Reduces background signal for improved polarization read accuracy.
Molecular Devices Fluorescence Filter, 700 nm, 60 nm Bandwidth, Holder
Molecular Devices fluorescence emission filter with holder (MPN 6590-0114) provides selective far-red detection at a 700 nm center wavelength with a 60 nm optical bandwidth. It is sold as a replaceable accessory for compatible multi-mode microplate readers and mounts in the instrument's filter holder for reliable signal selection.
- Center wavelength: 700 nm.
- Bandwidth: 60 nm.
- Filter type: emission filter for far-red fluorescence.
- Includes holder for instrument mounting.
- Typical application: far-red fluorescence assays on compatible readers.
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Molecular Devices Absorbance Filter, 450 nm, 8 nm, FilterMax Compatible
Replaceable narrow-band absorbance (photometric) filter designed for use in FilterMax multimode microplate readers. The filter is centered at 450 nm and has an approximate 8 nm bandwidth, providing a specific narrow bandpass for fixed-wavelength absorbance measurements in microplates.
- Center wavelength: 450 nm.
- Bandwidth: 8 nm (approximate).
- Designed for fixed-wavelength absorbance assays.
- Installs in the instrument filter wheel for modular wavelength selection.
- Narrow-bandpass optical filter for consistent photometric readings.
Molecular Devices Fluorescence Polarization Filter, 700 nm, Excitation
Molecular Devices 700 nm fluorescence polarization filter designed for fluorescence polarization (FP) microplate assays and near-infrared fluorescence detection. It provides excitation and detection at 700 nm for compatible multi-mode microplate readers.
- Provides excitation and detection centered at 700 nm for fluorescence polarization assays.
- Optimized for fluorescence polarization measurements in microplate formats.
- Enables near-infrared fluorescence detection to reduce background and improve signal-to-noise.
- Designed for easy installation in compatible filter holders and cartridges.
- Suitable for use with multi-mode microplate readers that support user-installable filters.
Molecular Devices Excitation Filter, 585 nm, 10 nm Bandwidth
This excitation filter provides narrowband fluorescence excitation centered at 585 nm with a 10 nm full width, for use in compatible multi-mode microplate readers.
- Center wavelength 585 nm.
- Bandwidth 10 nm full width.
- Excitation filter type suitable for narrowband fluorescence assays.
- Designed for installation in standard filter slide assemblies.
Molecular Devices Multi-Mode Microplate Reader, Five-Mode, HTRF Compatible, 6-384 Wells
The SpectraMax iD5 is a compact, five-mode multi-mode microplate reader designed for flexible bench-top detection of absorbance, fluorescence, luminescence, time-resolved fluorescence, and fluorescence polarization assays. The reader supports optional modules for HTRF and TR-FRET, bottom-read luminescence, and injector-based flash assays, and accepts 6-384 well plates.
- Five built-in detection modes for absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization.
- Expandable detection for HTRF, TR-FRET, BRET, and bottom-read luminescence with optional modules.
- Supports 6-384 well plate formats for endpoint and kinetic assays.
- Xenon flash light source with PMT and photodiode detectors for broad wavelength coverage.
- Temperature control to support assays up to 66°C and multiple shaking modes for assay mixing.
- Robotic compatibility for high-throughput automation workflows.
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Molecular Devices Absorbance Filter, 600 nm, 8 nm Bandwidth
The Molecular Devices absorbance bandpass filter centered at 600 nm with an 8 nm full width at half maximum (FWHM) bandwidth is a replacement optical filter for compatible microplate readers. It enables narrow-band absorbance measurements for endpoint and kinetic assays and installs as an interchangeable filter slide.
- Enables narrow-band absorbance measurements at 600 nm.
- 8 nm full width at half maximum bandpass for spectral selectivity.
- Designed for installation as an interchangeable filter slide in compatible microplate readers.
- Suitable for quantitative absorbance assays and endpoint or kinetic measurements.
- Replacement spare for instruments that accept interchangeable absorbance filters.
Molecular Devices Fluorescence Emission Filter, 660 nm, 32 nm Bandwidth
This emission filter is centered at 660 nm with a 32 nm full bandwidth and is intended for use in filter-based fluorescence microplate readers to isolate red emission wavelengths for assays using long-wavelength fluorophores.
- Center wavelength: 660 nm.
- Bandwidth (full width at half maximum): 32 nm.
- Product type: fluorescence emission filter for microplate readers.
- Application: isolates red emission for assays using red fluorophores and dyes.
- Benefit: improves spectral selectivity and reduces signal crosstalk between channels.
Molecular Devices Filter Lens, 550 nm, EMax and VMax Compatible
Replacement optical filter lens with a center wavelength of 550 nm for use in microplate readers. Designed as a field-replaceable spare to restore or modify detection for assays that require a 550 nm bandpass. Supplied as a single unit for straightforward replacement.
- Center wavelength 550 nm.
- Compatible with EMax and VMax microplate readers.
- Single replacement filter, sold each.
- Suitable for absorbance and fluorescence measurements requiring a 550 nm bandpass.